USP13 Antibody (OTI4G11)

Novus Biologicals | Catalog # NBP1-48047

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4G11
View all USP13 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human USP13 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

97.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP13 Antibody (OTI4G11)

Western Blot: USP13 Antibody (OTI4G11) [NBP1-48047]

Western Blot: USP13 Antibody (OTI4G11) [NBP1-48047]

Western Blot: USP13 Antibody (OTI4G11) [NBP1-48047] - Analysis of extracts (35ug) from 9 different cell lines by using anti-USP13 monoclonal antibody.
Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded prostate tissue using anti-USP13 mouse monoclonal antibody.
Western Blot: USP13 Antibody (OTI4G11) [NBP1-48047]

Western Blot: USP13 Antibody (OTI4G11) [NBP1-48047]

Western Blot: USP13 Antibody (OTI4G11) [NBP1-48047] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP13 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP13.
Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded breast tissue using anti-USP13 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-USP13 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded colon tissue using anti-USP13 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded Kidney tissue using anti-USP13mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded liver tissue using anti-USP13 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded lung tissue using anti-USP13 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded Ovary tissue using anti-USP13 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunohistochemistry-Paraffin: USP13 Antibody (OTI4G11) [NBP1-48047] - Staining of paraffin-embedded pancreas tissue using anti-USP13 mouse monoclonal antibody.
Immunoprecipitation: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunoprecipitation: USP13 Antibody (OTI4G11) [NBP1-48047]

Immunoprecipitation: USP13 Antibody (OTI4G11) [NBP1-48047] - (Negative control: IP without adding anti-USP13 antibody.). For each experiment, 500ul of DDK tagged USP13 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-USP13 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for USP13 Antibody (OTI4G11)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

4ug/ml

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.41 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP13

Long Name

Ubiquitin Specific Protease 13

Alternate Names

Isopeptidase T-3, IsoT-3, Ubiquitin Thioesterase 13

Entrez Gene IDs

8975 (Human)

Gene Symbol

USP13

Additional USP13 Products

Product Documents for USP13 Antibody (OTI4G11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for USP13 Antibody (OTI4G11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Customer Reviews for USP13 Antibody (OTI4G11)

There are currently no reviews for this product. Be the first to review USP13 Antibody (OTI4G11) and earn rewards!

Have you used USP13 Antibody (OTI4G11)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies