USP13 Antibody (OTI4G11) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-74802
![Western Blot: USP13 Antibody (OTI4G11)Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Western-Blot-NBP2-74802-img0002.jpg "Western Blot: USP13 Antibody (OTI4G11)Azide and BSA Free [NBP2-74802]")
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4G11
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Full-length protein expressed in 293T cell transfected with human USP13 expression vector
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
97.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for USP13 Antibody (OTI4G11) - Azide and BSA Free
Western Blot: USP13 Antibody (OTI4G11)Azide and BSA Free [NBP2-74802]
Western Blot: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP13 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP13.![Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74802-img0011.jpg "Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Staining of paraffin-embedded prostate tissue using anti-USP13 mouse monoclonal antibody.![Western Blot: USP13 Antibody (OTI4G11)Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Western-Blot-NBP2-74802-img0001.jpg "Western Blot: USP13 Antibody (OTI4G11)Azide and BSA Free [NBP2-74802]")
Western Blot: USP13 Antibody (OTI4G11)Azide and BSA Free [NBP2-74802]
Western Blot: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Analysis of extracts (35ug) from 9 different cell lines by using anti-USP13 monoclonal antibody.![Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74802-img0003.jpg "Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Staining of paraffin-embedded breast tissue using anti-USP13 mouse monoclonal antibody.![Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74802-img0004.jpg "Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-USP13 mouse monoclonal antibody.![Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74802-img0005.jpg "Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Staining of paraffin-embedded colon tissue using anti-USP13 mouse monoclonal antibody.![Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74802-img0006.jpg "Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Staining of paraffin-embedded Kidney tissue using anti-USP13mouse monoclonal antibody.![Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74802-img0007.jpg "Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Staining of paraffin-embedded liver tissue using anti-USP13 mouse monoclonal antibody.![Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74802-img0008.jpg "Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Staining of paraffin-embedded lung tissue using anti-USP13 mouse monoclonal antibody.![Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74802-img0009.jpg "Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Staining of paraffin-embedded Ovary tissue using anti-USP13 mouse monoclonal antibody.![Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74802-img0010.jpg "Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunohistochemistry: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - Staining of paraffin-embedded pancreas tissue using anti-USP13 mouse monoclonal antibody.![Immunoprecipitation: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]](https://resources.rndsystems.com/images/products/USP13-Antibody-OTI4G11-Azide-and-BSA-Free-Immunoprecipitation-NBP2-74802-img0012.jpg "Immunoprecipitation: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]")
Immunoprecipitation: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802]
Immunoprecipitation: USP13 Antibody (OTI4G11) - Azide and BSA Free [NBP2-74802] - (Negative control: IP without adding anti-USP13 antibody.). For each experiment, 500ul of DDK tagged USP13 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-USP13 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.Applications for USP13 Antibody (OTI4G11) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50
Immunoprecipitation
4ug/ml
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: USP13
Long Name
Ubiquitin Specific Protease 13
Alternate Names
Isopeptidase T-3, IsoT-3, Ubiquitin Thioesterase 13
Gene Symbol
USP13
Additional USP13 Products
Product Documents for USP13 Antibody (OTI4G11) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for USP13 Antibody (OTI4G11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for USP13 Antibody (OTI4G11) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review USP13 Antibody (OTI4G11) - Azide and BSA Free and earn rewards!
Have you used USP13 Antibody (OTI4G11) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...