USP14 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86946

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all USP14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP14 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: USP14 Antibody [NBP1-86946]

Immunocytochemistry/ Immunofluorescence: USP14 Antibody [NBP1-86946]

Immunocytochemistry/Immunofluorescence: USP14 Antibody [NBP1-86946] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
USP14 Antibody

Immunohistochemistry-Paraffin: USP14 Antibody [NBP1-86946] -

Immunohistochemistry-Paraffin: USP14 Antibody [NBP1-86946] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
USP14 Antibody

Immunohistochemistry-Paraffin: USP14 Antibody [NBP1-86946] -

Immunohistochemistry-Paraffin: USP14 Antibody [NBP1-86946] - Staining of human lymph node shows strong cytoplasmic and membranous positivity in germinal center cells.
USP14 Antibody

Immunohistochemistry-Paraffin: USP14 Antibody [NBP1-86946] -

Immunohistochemistry-Paraffin: USP14 Antibody [NBP1-86946] - Staining of human cerebral cortex shows moderate cytoplasmic and membranous positivity in neurons.
USP14 Antibody - BSA Free Western Blot: USP14 Antibody - BSA Free [NBP1-86946]

Western Blot: USP14 Antibody - BSA Free [NBP1-86946]

Analysis in human cell line U-251 MG.
USP14 Antibody - BSA Free Immunohistochemistry-Paraffin: USP14 Antibody - BSA Free [NBP1-86946]

Immunohistochemistry-Paraffin: USP14 Antibody - BSA Free [NBP1-86946]

Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
USP14 Antibody - BSA Free Western Blot: USP14 Antibody - BSA Free [NBP1-86946]

Western Blot: USP14 Antibody - BSA Free [NBP1-86946]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for USP14 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP14

USP14 encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Ubiquitin Specific Protease 14

Alternate Names

TGT, Ubiquitin Thioesterase 14, Ubp6

Entrez Gene IDs

9097 (Human)

Gene Symbol

USP14

UniProt

P54578

Additional USP14 Products

Product Documents for USP14 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for USP14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for USP14 Antibody - BSA Free

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Protocols

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