USP19 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93529

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all USP19 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human USP19 (NP_001186089.1). MSGGASATGPRRGPPGLEDTTSKKKQKDRANQESKDGDPRKETGSRYVAQAGLEPLASGDPSASASHAAGITGSRHRTRLFFPSSSGSASTPQEEQTKEGACEDPHDLLATPTPELLLDWRQSAEEVIVKLRVGVGPLQLEDVDAAFTDTDCVVRFAGGQQWGGVFYAEIKSSCAKVQTRKGSLLHLTLPKKVPMLTWPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit USP19 Antibody - Azide and BSA Free (NBP2-93529) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for USP19 Antibody - Azide and BSA Free

Western Blot: USP19 AntibodyAzide and BSA Free [NBP2-93529]

Western Blot: USP19 AntibodyAzide and BSA Free [NBP2-93529]

Western Blot: USP19 Antibody [NBP2-93529] - Analysis of extracts of various cell lines, using USP19 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
USP19 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: USP19 Antibody - Azide and BSA Free [NBP2-93529] -

Immunocytochemistry/ Immunofluorescence: USP19 Antibody - Azide and BSA Free [NBP2-93529] - Immunofluorescence analysis of L929 cells using USP19 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for USP19 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP19

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 19 (USP19) is a member of this family. USP19 is a 146 kDa protein that contains two CS domains and one MYND-type zinc finger.

Long Name

Ubiquitin Specific Protease 19

Alternate Names

Ubiquitin Carboxyl-Terminal Hydrolase 19, Ubiquitin Thiolesterase 19, ZMYND9

Gene Symbol

USP19

Additional USP19 Products

Product Documents for USP19 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for USP19 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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Protocols

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