USP20 Antibody

Novus Biologicals | Catalog # NB100-1260

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all USP20 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HGEQKIEAETRAV, from the C Terminus of the protein sequence according to NP_006667.3.

Specificity

Reported variants represent identical protein (NP_006667.3; NP_001103773.2; NP_001008563.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for USP20 Antibody

Western Blot: USP20 Antibody [NB100-1260]

Western Blot: USP20 Antibody [NB100-1260]

Western Blot: USP20 Antibody [NB100-1260] - staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: USP20 Antibody [NB100-1260]

Immunohistochemistry-Paraffin: USP20 Antibody [NB100-1260]

Immunohistochemistry-Paraffin: USP20 Antibody [NB100-1260] - (3ug/ml) IHC analysis of USP20 in paraffin embedded Human Lymph Node using this antibody at 3 ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Applications for USP20 Antibody

Application
Recommended Usage

Immunohistochemistry

3 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Peptide ELISA

detection limit 1:32000

Western Blot

1:100-1:2000
Application Notes
WB: Approx. 100 kDa band observed in human tonsil lysates (calculated MW of 102 kDa band according to NP_006667.3).
IHC-P: n paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Data obtained from a previous batch (different goat).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP20

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Long Name

Ubiquitin Specific Protease 20

Alternate Names

LSFR3A, Ubiquitin Thiolesterase 20, VDU2

Entrez Gene IDs

10868 (Human); 74270 (Mouse)

Gene Symbol

USP20

UniProt

NP_006667. (Human)

Additional USP20 Products

Product Documents for USP20 Antibody

Certificate of Analysis

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Product Specific Notices for USP20 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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