USP26 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93692

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 664-913 of human USP26 (NP_114113.1). TSLCQFHKAGGKPASSPGTPLSKVDFQTVPENPKRKKYVKTSKFVAFDRIINPTKDLYEDKNIRIPERFQKVSEQTQQCDGMRICEQAPQQALPQSFPKPGTQGHTKNLLRPTKLNLQKSNRNSLLALGSNKNPRNKDILDKIKSKAKETKRNDDKGDHTYRLISVVSHLGKTLKSGHYICDAYDFEKQIWFTYDDMRVLGIQEAQMQEDRRCTGYIFFYMHNEIFEEMLKREENAQLNSKEVEETLQKE

Reactivity Notes

Use in Human reported in scientific literature (PMID:34202084).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit USP26 Antibody - BSA Free (NBP2-93692) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-USP26 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for USP26 Antibody - BSA Free

Western Blot: USP26 AntibodyAzide and BSA Free [NBP2-93692]

Western Blot: USP26 AntibodyAzide and BSA Free [NBP2-93692]

Western Blot: USP26 Antibody [NBP2-93692] - Analysis of extracts of various cell lines, using USP26 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
USP26 Antibody - BSA Free

Western Blot: USP26 Antibody - BSA Free [NBP2-93692] -

Expression analysis of USP26 mRNA and USP26 in the spermatozoa from a normal male control and men harboring hemizygous USP26 variants. (A) Real-time quantitative PCR analysis indicated that the abundance of USP26 mRNA was significantly reduced in the sperm from men harboring hemizygous USP26 variants, when compared to that of a normal control man. Data represent the means +/- standard error of measurement of three independent experiments. Two-tailed Student’s paired or unpaired t-tests were used as appropriate (*** p < 0.001). (B) Immunoblotting assay indicated dramatically reduced expression of USP26 in the spermatozoa from men harboring hemizygous USP26 variants. beta -actin was used as a loading control. The red circle represents non-specific bands. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34202084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for USP26 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:500-1:2000
Application Notes
Use in WB, ICC/IF reported in scientific literature (PMID:34202084)

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP26

The USP26 gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases and is a deubiquitinatingenzyme (DUB) with His and Cys domains. It is specifically expressed in testis tissue. Mutations in this gene have beenassociated with Sertol

Long Name

Ubiquitin Specific Protease 26

Alternate Names

Deubiquitinating enzyme 26, EC 3.4.19.12, MGC120066, MGC120067, MGC120068, ubiquitin carboxyl-terminal hydrolase 26, ubiquitin specific peptidase 26, ubiquitin specific protease 26, ubiquitin thioesterase 26, Ubiquitin thiolesterase 26, ubiquitin-specific processing protease 26, Ubiquitin-specific-processing protease 26

Gene Symbol

USP26

Additional USP26 Products

Product Documents for USP26 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for USP26 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for USP26 Antibody - BSA Free

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Protocols

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