V-type proton ATPase subunit F Antibody (OTI1B8)

Novus Biologicals | Catalog # NBP2-03498

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1B8
View all V-type proton ATPase subunit F Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

13.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for V-type proton ATPase subunit F Antibody (OTI1B8)

Western Blot: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Western Blot: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Western Blot: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY V-type proton ATPase subunit F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-V-type proton ATPase subunit F.
Immunocytochemistry/Immunofluorescence: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] Staining of COS7 cells transiently transfected by pCMV6-ENTRY V-type proton ATPase subunit F.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human thyroid tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Flow Cytometry: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Flow Cytometry: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Flow Cytometry: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-V-type proton ATPase subunit F antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human Kidney tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human liver tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lung tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lymph node tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (OTI1B8) [NBP2-03498]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Applications for V-type proton ATPase subunit F Antibody (OTI1B8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: V-type proton ATPase subunit F

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq].

Alternate Names

ATP6S14adenosinetriphosphatase 14k chain, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F, H(+)-transporting two-sector ATPase, 14kD subunit, MGC117321, MGC126037, MGC126038, vacuolar ATP synthase subunit F, vacuolar proton pump F subunit, Vacuolar proton pump subunit F, VATFATPase, vacuolar, 14 kD, V-ATPase 14 kDa subunit, V-ATPase F subunit, V-ATPase subunit F, Vma7, V-type proton ATPase subunit F

Entrez Gene IDs

9296 (Human)

Gene Symbol

ATP6V1F

Additional V-type proton ATPase subunit F Products

Product Documents for V-type proton ATPase subunit F Antibody (OTI1B8)

Certificate of Analysis

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Product Specific Notices for V-type proton ATPase subunit F Antibody (OTI1B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for V-type proton ATPase subunit F Antibody (OTI1B8)

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Protocols

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