VAMP3/Cellubrevin Antibody

Novus Biologicals | Catalog # NB300-510

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Mouse, Canine

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all VAMP3/Cellubrevin Primary Antibodies »

Product Specifications

Immunogen

Sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VAMP3/Cellubrevin Antibody

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510]

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510]

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of 25 ug of mouse brain (lane 1), rat brain (lane 2) and human brain (lane 3) lysates.
Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510]

Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510]

Immunocytochemistry/Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of C6 cells (right) compared to a negative control without primary antibody (left).
Western Blot: VAMP3/Cellubrevin Antibody [NB300-510]

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510]

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of recombinant rat VAMP-3.
Western Blot: VAMP3/Cellubrevin Antibody [NB300-510]

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510]

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP-3 in hela cell extract.
Western Blot: VAMP3/Cellubrevin Antibody [NB300-510]

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510]

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of HeLa cell lysates.
Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510]

Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510]

Immunocytochemistry/Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510]

Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510]

Immunocytochemistry/Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of Neuro-2a cells (right) compared to a negative control without primary antibody (left).

Applications for VAMP3/Cellubrevin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Western Blot

1:1000 - 1:5000
Application Notes
May be useful in WB. WB: Detects a 13 kDa protein (predicted MW 12 kDa). ICC/IF usage was reported in scientific literature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAMP3/Cellubrevin

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, all VAMP isoform levels appear to be significantly reduced.

Alternate Names

CEBCellubrevin, cellubrevin, SYB3, synaptobrevin-3, VAMP-3, vesicle-associated membrane protein 3, vesicle-associated membrane protein 3 (cellubrevin)

Entrez Gene IDs

9341 (Human); 22319 (Mouse)

Gene Symbol

VAMP3

UniProt

P63024 (Mouse)

Additional VAMP3/Cellubrevin Products

Product Documents for VAMP3/Cellubrevin Antibody

Certificate of Analysis

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Product Specific Notices for VAMP3/Cellubrevin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VAMP3/Cellubrevin Antibody

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Protocols

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