VAP-1/AOC3 Antibody (174-5) - Azide Free

Novus Biologicals | Catalog # NBP1-58375

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 174-5

Format

Azide Free
View all VAP-1/AOC3 Primary Antibodies »

Product Specifications

Immunogen

The monoclonal antibody 174-5 cross-reacts with rat VAP-1. The monoclonal antibody inhibits lymphocyte infiltration in liver allograft rejection in rats.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VAP-1/AOC3 Antibody (174-5) - Azide Free

Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody (174-5) [NBP1-58375]

Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody (174-5) [NBP1-58375]

Immunohistochemistry-Paraffin: VAP1 Antibody (174-5) [NBP1-58375] - Analysis of VAP1 on human tonsil tissue. Anti-human VAP1 staining results in vessels that are VAP1 positive, whereas morphologically similar vessels next to postive ones can be negative.

Applications for VAP-1/AOC3 Antibody (174-5) - Azide Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: VAP-1/AOC3

The monoclonal antibody 174-5 recognises human Vascular Adhesion Protein-1 (VAP-1) also known as AOC3, a heavily sialylated homodimeric glycoprotein of 180 kD. VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. The majority of VAP-1 is stored within intracellular granules and translocated to the endothelial surface on inflammation. Structurally it belongs to enzymes called semicarbamizide-sensitive amine oxidases. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 possesses both adhesive and enzymatic properties in vitro. VAP-1 mediates leukocyte trafficking to sites of inflammation. The monoclonal antibody 174-5 cross-reacts with rat VAP-1. The monoclonal antibody inhibits lymphocyte infiltration in liver allograft rejection in rats.

Long Name

Vascular Adhesion Protein-1

Alternate Names

AOC3, HPAO, SSAO, VAP1

Gene Symbol

AOC3

Additional VAP-1/AOC3 Products

Product Documents for VAP-1/AOC3 Antibody (174-5) - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VAP-1/AOC3 Antibody (174-5) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VAP-1/AOC3 Antibody (174-5) - Azide Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VAP-1/AOC3 Antibody (174-5) - Azide Free

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  • Q: One of our customers is very interested in your Antibody: VAP1 Antibody (174-5). This antibody is not listed as functional assays, but in the reference cited in the data sheet, there is an article referring this antibody as blocking VAP-1 in vivo in rats. The article is: Martelius, T et al. Blockade of vascular adhesion protein-1 inhibits lymphocyte infiltration in rat liver allograft rejection. Am J Path 2004, 165: 199. Could you please confirm that this antibody could be used in Functional assays? Also the format is really small and the customer need huge quantity of antibody as the recommended dosys is 2mg/kg. Do you have any other bigger format?

    A: This antibody has not been tested in functional assays. It has been validated for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry-Frozen. The reference you refer to has been taken off our website. I apologize for any confusion.

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