VASA Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20844

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Fish

Predicted:

Bovine (94%), Porcine (98%), Primate (100%), Rat (90%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VASA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VASA. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%), Siberian sturgeon (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit VASA Antibody - BSA Free (NBP2-20844) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for VASA Antibody - BSA Free

Western Blot: VASA Antibody [NBP2-20844]

Western Blot: VASA Antibody [NBP2-20844]

Western Blot: VASA Antibody [NBP2-20844] - Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
VASA Antibody

Western Blot: VASA Antibody [NBP2-20844] -

Western Blot: VASA Antibody [NBP2-20844] - Mouse tissue extract (30 | 50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VASA antibody [C1C3] (NBP2-20844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
VASA Antibody

Western Blot: VASA Antibody [NBP2-20844] -

Western Blot: VASA Antibody [NBP2-20844] - Human tissue extracts (30 | 50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VASA antibody [C1C3] (NBP2-20844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for VASA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

1:10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VASA

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq]

Long Name

DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41

Alternate Names

DDX4

Gene Symbol

DDX4

UniProt

Q9NQI0

Additional VASA Products

Product Documents for VASA Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VASA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Primordial and Mature Germ Cells

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Protocols

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FAQs

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