VASP Antibody (OTI4D6)

Novus Biologicals | Catalog # NBP2-00555

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D6
View all VASP Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human VASP(NP_003361) produced in 293T Cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Focal Adhesion Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VASP Antibody (OTI4D6)

Western Blot: VASP Antibody (OTI4D6) [NBP2-00555]

Western Blot: VASP Antibody (OTI4D6) [NBP2-00555]

Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: VASP Antibody (OTI4D6) [NBP2-00555]

Immunocytochemistry/ Immunofluorescence: VASP Antibody (OTI4D6) [NBP2-00555]

Immunocytochemistry/Immunofluorescence: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human lymphoma tissue using anti-VASP mouse monoclonal antibody.
Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555]

Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555]

Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of Jurkat cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: VASP Antibody (OTI4D6) [NBP2-00555]

Western Blot: VASP Antibody (OTI4D6) [NBP2-00555]

Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human endometrium tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human liver tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555]

Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human lymph node tissue using anti-VASP mouse monoclonal antibody.
Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555]

Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555]

Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of Hela cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for VASP Antibody (OTI4D6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq].

Long Name

Vasodilator-stimulated Phosphoprotein

Alternate Names

vasodilator-stimulated phosphoprotein

Entrez Gene IDs

7408 (Human)

Gene Symbol

VASP

Additional VASP Products

Product Documents for VASP Antibody (OTI4D6)

Certificate of Analysis

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Product Specific Notices for VASP Antibody (OTI4D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Platelet Markers

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Protocols

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