Vav-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86867

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Vav-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSWTPIAQNRGIMPFPTEEESVGDEDIYSGLSDQIDDTVEEDEDLYDCVENEEAEGDEIYEDLMRSEPVSMPPKMTEYDKRCCCLREIQQTEEKYTDTLGSIQQHFLKPLQRFLKPQDIEIIFINIEDLLRVHTHFLKEMKEAL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Vav-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Vav-1 Antibody [NBP1-86867]

Immunocytochemistry/ Immunofluorescence: Vav-1 Antibody [NBP1-86867]

Immunocytochemistry/Immunofluorescence: Vav-1 Antibody [NBP1-86867] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Vav-1 Antibody - BSA Free Immunohistochemistry-Paraffin: Vav-1 Antibody - BSA Free [NBP1-86867]

Immunohistochemistry-Paraffin: Vav-1 Antibody - BSA Free [NBP1-86867]

Analysis in human lymph node and pancreas tissues Corresponding VAV1 RNA-seq data are presented for the same tissues.
Vav-1 Antibody - BSA Free Immunohistochemistry-Paraffin: Vav-1 Antibody - BSA Free [NBP1-86867]

Immunohistochemistry-Paraffin: Vav-1 Antibody - BSA Free [NBP1-86867]

Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: Vav-1 Antibody [NBP1-86867]

Immunohistochemistry-Paraffin: Vav-1 Antibody [NBP1-86867]

Immunohistochemistry-Paraffin: Vav-1 Antibody [NBP1-86867] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Vav-1 Antibody [NBP1-86867]

Immunohistochemistry-Paraffin: Vav-1 Antibody [NBP1-86867]

Immunohistochemistry-Paraffin: Vav-1 Antibody [NBP1-86867] - Staining of human skeletal muscle shows no positivity in myoctes as expected.
Immunohistochemistry-Paraffin: Vav-1 Antibody [NBP1-86867]

Immunohistochemistry-Paraffin: Vav-1 Antibody [NBP1-86867]

Immunohistochemistry-Paraffin: Vav-1 Antibody [NBP1-86867] - Staining of human prostate shows weak cytoplasmic positivity in smooth muscle cells.

Applications for Vav-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vav-1

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels (2). As a signaling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein (3). Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response (2).

Alternate Names

vav-T, Vav1

Gene Symbol

VAV1

Additional Vav-1 Products

Product Documents for Vav-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Vav-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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