VDAC1 Antibody (3A1) - BSA Free

Novus Biologicals | Catalog # NBP3-26256

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3A1 expressed in HEK293

Format

BSA Free
View all VDAC1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human VDAC1 [UniProt P21796]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VDAC1 Antibody (3A1) - BSA Free

VDAC1 Antibody (3A1)

Immunohistochemistry: VDAC1 Antibody (3A1) [NBP3-26256] -

Immunohistochemistry: VDAC1 Antibody (3A1) [NBP3-26256] - Image of VDAC1 Antibody (3A1) diluted at 1:73.125 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
VDAC1 Antibody (3A1)

Immunohistochemistry: VDAC1 Antibody (3A1) [NBP3-26256] -

Immunohistochemistry: VDAC1 Antibody (3A1) [NBP3-26256] - Image of VDAC1 Antibody (3A1) diluted at 1:73.125 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
VDAC1 Antibody (3A1)

Western Blot: VDAC1 Antibody (3A1) [NBP3-26256] -

Western Blot: VDAC1 Antibody (3A1) [NBP3-26256] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, LO2 whole cell lysate, Raji whole cell lysate, Rat heart tissue, Mouse brain tissue.
All lanes: VDAC1 Antibody at 0.7ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 31 KDa
Observed band size: 31 KDa

Applications for VDAC1 Antibody (3A1) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: VDAC1

VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.

Long Name

Voltage-dependent anion-selective channel protein 1

Alternate Names

hVDAC1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC, VDAC-1

Gene Symbol

VDAC1

Additional VDAC1 Products

Product Documents for VDAC1 Antibody (3A1) - BSA Free

Certificate of Analysis

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Product Specific Notices for VDAC1 Antibody (3A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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