VDP p115 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-17245

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VDP p115 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VDP p115. The exact sequence is proprietary.

Localization

Cytoplasm; cytosol; Golgi apparatus membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

108 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VDP p115 Antibody - BSA Free

Western Blot: VDP p115 Antibody [NBP2-17245]

Western Blot: VDP p115 Antibody [NBP2-17245]

Western Blot: VDP p115 Antibody [NBP2-17245] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: VDP p115 Antibody [NBP2-17245]

Immunohistochemistry-Paraffin: VDP p115 Antibody [NBP2-17245]

Immunohistochemistry-Paraffin: VDP p115 Antibody [NBP2-17245] - Immunohistochemical analysis of paraffin-embedded lung AdCA, using antibody at 1:500 dilution.
VDP p115 Antibody

Western Blot: VDP p115 Antibody [NBP2-17245] -

Western Blot: VDP p115 Antibody [NBP2-17245] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with VDP p115 antibody [N1N2], N-term (NBP2-17245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
VDP p115 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: VDP p115 Antibody [NBP2-17245] -

VDP / p115 antibody [N1N2], N-term detects VDP / p115 protein at cytoplasm and golgi apparatus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: VDP / p115 stained by VDP / p115 antibody [N1N2], N-term (NBP2-17245) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.

Applications for VDP p115 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VDP p115

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner. [provided by RefSeq]

Alternate Names

p115, Transcytosis-associated protein, USO1 homolog, vesicle docking protein (yeast), USO1 vesicle docking protein homolog (yeast), vesicle docking protein, vesicle docking protein p115

Gene Symbol

USO1

Additional VDP p115 Products

Product Documents for VDP p115 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VDP p115 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for VDP p115 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review VDP p115 Antibody - BSA Free and earn rewards!

Have you used VDP p115 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies