VDR/NR1I1/Vitamin D Receptor Antibody (3C7) - BSA Free

Novus Biologicals | Catalog # NBP3-26255

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3C7 expressed in HEK293

Format

BSA Free
View all VDR/NR1I1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human VDR/NR1I1/Vitamin D Receptor [UniProt P11473]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VDR/NR1I1/Vitamin D Receptor Antibody (3C7) - BSA Free

VDR/NR1I1/Vitamin D Receptor Antibody (3C7)

Immunohistochemistry: VDR/NR1I1/Vitamin D Receptor Antibody (3C7) [NBP3-26255] -

Immunohistochemistry: VDR/NR1I1/Vitamin D Receptor Antibody (3C7) [NBP3-26255] - Image of VDR/NR1I1/Vitamin D Receptor Antibody (3C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
VDR/NR1I1/Vitamin D Receptor Antibody (3C7)

Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody (3C7) [NBP3-26255] -

Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody (3C7) [NBP3-26255] - Positive Western Blot detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, HL-60 whole cell lysate.
All lanes: VDR/NR1I1/Vitamin D Receptor Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 49, 54 kDa
Observed band size: 49 kDa

Applications for VDR/NR1I1/Vitamin D Receptor Antibody (3C7) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: VDR/NR1I1

Vitamin D Receptor encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein.

Long Name

Vitamin D [1,25-Dihydroxyvitamin D3] Receptor

Alternate Names

NR1I1

Gene Symbol

VDR

Additional VDR/NR1I1 Products

Product Documents for VDR/NR1I1/Vitamin D Receptor Antibody (3C7) - BSA Free

Certificate of Analysis

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Product Specific Notices for VDR/NR1I1/Vitamin D Receptor Antibody (3C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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