VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16)

Novus Biologicals | Catalog # NBP2-66778

Recombinant Monoclonal Antibody
Novus Biologicals
Loading...

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA11-16 expressed in HEK293
Loading...

Product Specifications

Immunogen

Synthetic peptide with Human VDR/NR1I1/Vitamin D Receptor aa 1-50 / 427. (SwissProt: P11473 Human; SwissProt: P48281 Mouse; SwissProt: P13053 Rat)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16)

Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Western blot analysis of VDR/NR1I1/Vitamin D Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: U937 cell lysate
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded rat skin tissue with Rabbit anti-Vitamin D Receptor antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Flow Cytometry: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Flow Cytometry: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of Hela cells with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse colon tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded human skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded rat colon tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Vitamin D Receptor antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VDR/NR1I1

Vitamin D Receptor encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein.

Long Name

Vitamin D [1,25-Dihydroxyvitamin D3] Receptor

Alternate Names

NR1I1

Gene Symbol

VDR

Additional VDR/NR1I1 Products

Product Documents for VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16)

There are currently no reviews for this product. Be the first to review VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) and earn rewards!

Have you used VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...