VEGFR2/KDR/Flk-1 Antibody (001) - Azide and BSA Free

Name: VEGFR2/KDR/Flk-1 Antibody (001) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-89199
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-89199

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Sandwich ELISA Capture, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001 expressed in HEK293

Format

Azide and BSA Free

View all VEGFR2/KDR/Flk-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human VEGFR2/KDR/Flk-1 (Accession#: NP_002244.1; Met1-Glu764).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for VEGFR2/KDR/Flk-1 Antibody (001) - Azide and BSA Free

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (001) [NBP2-89199]

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (001) [NBP2-89199] - Analysis of Human VEGFR2(CD309) expression on HUVEC cells. Cells were stained with purified anti-Human VEGFR2(CD309), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for VEGFR2/KDR/Flk-1 Antibody (001) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1:25-1:100

Sandwich ELISA Capture

1:250-1:2000

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR2/KDR/Flk-1

Vascular endothelial growth factor receptor 2 (VEGFR2) is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other common growth factor receptors, VEGF receptor 2 dimerises upon ligand binding and is autophosphorylated at multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or can serve as binding sites for SH2 and phosphotyrosine binding containing signaling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Defects in the VEGFR2 gene are associated with susceptibility to benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium called hemangioma capillary infantile. HCI is the most common tumor found in infants, found in up to 10% of all births.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Gene Symbol

KDR

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for VEGFR2/KDR/Flk-1 Antibody (001) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for VEGFR2/KDR/Flk-1 Antibody (001) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.