VGF Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-31596

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VGF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: APARDELPDWNEVLPPWDREEDEVYPPGPYHPFPNYIRPRTLQPPSALRRRHYHHALPPSRHYPGREAQARRAQEEAEAEERRLQEQEELENYIEHV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VGF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: VGF Antibody [NBP2-31596]

Immunocytochemistry/ Immunofluorescence: VGF Antibody [NBP2-31596]

Immunocytochemistry/Immunofluorescence: VGF Antibody [NBP2-31596] - Staining of human cell line SK-MEL-30 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human pituitary gland shows strong cytoplasmic positivity in anterior cells.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining in human cerebral cortex and liver tissues using anti-VGF antibody. Corresponding VGF RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human colon.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human testis.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Analysis in human cerebral cortex and prostate tissues. Corresponding VGF RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human hypothalamus, pituitary gland, placenta and prostate using Anti-VGF antibody NBP2-31596 (A) shows similar protein distribution across tissues to independent antibody NBP2-56358 (B).
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human hypothalamus shows strong positivity in a subset of neurons.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human pituitary gland shows strong positivity in anterior cells.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596]

Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human placenta shows no positivity in trophoblastic cells as expected.

Applications for VGF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VGF

Nerve growth factor (NGF) is a peptide that plays a key role in the differentiation and survival of neurons in the peripheral nervous system (PNS) and the central nervous system (CNS). VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line. VGF is widely expressed in both the PNS and CNS, but is especially abundant in the adult hypothalamus. VGF plays an essential role in how the brain regulates energy expenditure and body weight. Its expression is rapidly induced by injury, the circadian clock, and neuronal activity.

Long Name

Neurosecretory Protein VGF

Alternate Names

neuro-endocrine specific protein VGF, neurosecretory protein VGF, VGF nerve growth factor inducible

Gene Symbol

VGF

UniProt

O15240

Additional VGF Products

Product Documents for VGF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VGF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Nutrition and Appetite

Customer Reviews for VGF Antibody - BSA Free

There are currently no reviews for this product. Be the first to review VGF Antibody - BSA Free and earn rewards!

Have you used VGF Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies