VGLUT2 Antibody (CL2921)
Novus Biologicals | Catalog # NBP3-52070
Recombinant Monoclonal Antibody
![VGLUT2 Antibody (CL2921) Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]](https://resources.rndsystems.com/images/products/nbp3-52070_mouse-vglut2-mab-cl2921-immunohistochemistry-273202615235220.jpg "Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL2921
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q9P2U8, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for VGLUT2 Antibody (CL2921)
Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]
Staining of human tonsil shows no positivity in lymphoid cells as expected.![VGLUT2 Antibody (CL2921) Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]](https://resources.rndsystems.com/images/products/nbp3-52070_mouse-vglut2-mab-cl2921-immunohistochemistry-27320261527557.jpg "Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]")
Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]
Staining of rat brain shows moderate to strong positivity in glutamatergic fibers in the cerebellum.![VGLUT2 Antibody (CL2921) Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]](https://resources.rndsystems.com/images/products/nbp3-52070_mouse-vglut2-mab-cl2921-immunohistochemistry-27320261523322.jpg "Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]")
Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]
Staining of mouse brain shows positivity in glutamatergic fibers in the cerebellum.![VGLUT2 Antibody (CL2921) Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]](https://resources.rndsystems.com/images/products/nbp3-52070_mouse-vglut2-mab-cl2921-immunohistochemistry-27320261527550.jpg "Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]")
Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]
Staining of human cerebellum shows moderate to strong positivity in glutamatergic fibers.![VGLUT2 Antibody (CL2921) Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]](https://resources.rndsystems.com/images/products/nbp3-52070_mouse-vglut2-mab-cl2921-immunohistochemistry-27320261520456.jpg "Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]")
Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]
Staining of rat hippocampus shows moderate positivity in glutamatergic fibers in granular cell layer of the dentate gyrus.![VGLUT2 Antibody (CL2921) Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]](https://resources.rndsystems.com/images/products/nbp3-52070_mouse-vglut2-mab-cl2921-immunohistochemistry-2732026153007.jpg "Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]")
Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]
Staining of mouse brain shows strong positivity in glutamatergic fibers in the thalamus.![VGLUT2 Antibody (CL2921) Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]](https://resources.rndsystems.com/images/products/nbp3-52070_mouse-vglut2-mab-cl2921-immunohistochemistry-27320261519610.jpg "Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]")
Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]
Staining of human cerebral cortex shows strong positivity in neuropil.![VGLUT2 Antibody (CL2921) Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]](https://resources.rndsystems.com/images/products/nbp3-52070_mouse-vglut2-mab-cl2921-immunohistochemistry-27320261527553.jpg "Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]")
Immunohistochemistry: VGLUT2 Antibody (CL2921) [NBP3-52070]
Analysis in human cerebral cortex and tonsil tissues. Corresponding VGLUT2 RNA-seq data are presented for the same tissues.Applications for VGLUT2 Antibody (CL2921)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VGLUT2
Alternate Names
differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, DNPI, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, solute carrier family 17 member 6, vesicular glutamate transporter 2, VGLUT2
Gene Symbol
SLC17A6
Additional VGLUT2 Products
Product Documents for VGLUT2 Antibody (CL2921)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VGLUT2 Antibody (CL2921)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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