VGLUT2 Antibody (CL2921)

Novus Biologicals | Catalog # NBP3-52463

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL2921
View all VGLUT2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9P2U8, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VGLUT2 Antibody (CL2921)

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Staining of human cerebellum shows moderate to strong positivity in glutamatergic fibers.
Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Staining of mouse brain shows positivity in glutamatergic fibers in the cerebellum.
Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Staining of mouse brain shows strong positivity in glutamatergic fibers in the thalamus.
Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Staining of rat brain shows moderate to strong positivity in glutamatergic fibers in the cerebellum.
Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Staining of rat hippocampus shows moderate positivity in glutamatergic fibers in granular cell layer of the dentate gyrus.

Applications for VGLUT2 Antibody (CL2921)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: VGLUT2

VGLUT2 is a sodium dependent inorganic phosphate cotransporter that is involved in the calcium dependent glutamate release from astrocytes. It is expressed in only a subset of neurons, localized to synaptic vesicles, at synapses exhibiting classical excitatory features. VGLUT2 mRNA is found in brain regions that lack VGLUT1, a related protein also displaying sodium dependent inorganic phosphate cotransporter activity.

Alternate Names

differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, DNPI, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, solute carrier family 17 member 6, vesicular glutamate transporter 2, VGLUT2

Gene Symbol

SLC17A6

Additional VGLUT2 Products

Product Documents for VGLUT2 Antibody (CL2921)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VGLUT2 Antibody (CL2921)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for VGLUT2 Antibody (CL2921)

There are currently no reviews for this product. Be the first to review VGLUT2 Antibody (CL2921) and earn rewards!

Have you used VGLUT2 Antibody (CL2921)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies