Vinculin Antibody (VCL/2575)

Novus Biologicals | Catalog # NBP2-79808

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # VCL/2575
View all Vinculin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary) (Uniprot: P18206)

Localization

Cytoplasmic

Marker

Marker of Age-related Macular Degeneration

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79942)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Vinculin Antibody (VCL/2575)

Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808]

Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808]

Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808] - Western Blot Analysis of human U-87, THP-1 cell lysate using Vinculin Antibody (VCL/2575).
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2575) [NBP2-79808]

Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2575) [NBP2-79808]

Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2575) [NBP2-79808] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Antibody (VCL/2575).
Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808]

Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808]

Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808] - Western Blot Analysis of human A431, K562, NIH3T3 cell lysate using Vinculin Antibody (VCL/2575).
Protein Array: Vinculin Antibody (VCL/2575) [NBP2-79808]

Protein Array: Vinculin Antibody (VCL/2575) [NBP2-79808]

Protein Array: Vinculin Antibody (VCL/2575) [NBP2-79808] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Antibody (VCL/2575) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Vinculin Antibody (VCL/2575)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Gene Symbol

VCL

UniProt

P18206

Additional Vinculin Products

Product Documents for Vinculin Antibody (VCL/2575)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Vinculin Antibody (VCL/2575)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Vinculin Antibody (VCL/2575)

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  • Q: Do you have any 100 coda or bigger controls?

    A:

    I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

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