Vitronectin Antibody (1G11E8) - BSA Free

Novus Biologicals | Catalog # NBP2-52586

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1G11E8

Format

BSA Free
View all Vitronectin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Vitronectin (AA: 20-199) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vitronectin Antibody (1G11E8) - BSA Free

Western Blot: Vitronectin Antibody (1G11E8)BSA Free [NBP2-52586]

Western Blot: Vitronectin Antibody (1G11E8)BSA Free [NBP2-52586]

Western Blot: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis using VTN mAb against human VTN (AA: 20-199) recombinant protein. (Expected MW is 45.9 kDa)
Immunohistochemistry-Paraffin: Vitronectin Antibody (1G11E8) - BSA Free [NBP2-52586]

Immunohistochemistry-Paraffin: Vitronectin Antibody (1G11E8) - BSA Free [NBP2-52586]

Immunohistochemistry-Paraffin: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis of paraffin-embedded liver cancer tissues using VTN mouse mAb with DAB staining.
Flow Cytometry: Vitronectin Antibody (1G11E8) - BSA Free [NBP2-52586]

Flow Cytometry: Vitronectin Antibody (1G11E8) - BSA Free [NBP2-52586]

Flow Cytometry: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis of Hela cells using VTN mouse mAb (green) and negative control (red).
Western Blot: Vitronectin Antibody (1G11E8)BSA Free [NBP2-52586]

Western Blot: Vitronectin Antibody (1G11E8)BSA Free [NBP2-52586]

Western Blot: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis using VTN mAb against HEK293 (1) and VTN (AA: 20-199)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Vitronectin Antibody (1G11E8) - BSA Free [NBP2-52586]

Immunohistochemistry-Paraffin: Vitronectin Antibody (1G11E8) - BSA Free [NBP2-52586]

Immunohistochemistry-Paraffin: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis of paraffin-embedded cervical cancer tissues using VTN mouse mAb with DAB staining.
ELISA: Vitronectin Antibody (1G11E8) - BSA Free [NBP2-52586]

ELISA: Vitronectin Antibody (1G11E8) - BSA Free [NBP2-52586]

ELISA: Vitronectin Antibody (1G11E8) [NBP2-52586] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for Vitronectin Antibody (1G11E8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vitronectin

Vitronectin also called serum spreading factor or complement S-protein, is a member of the pexin family (1). Found in serum and in tissues, Vitronectin exist in two forms; as a single chain (75kDa) and as a clipped form composed of two chains (65kDa and 10kDa). Vitronectin promotes cell adhesion, spreading and migration through specific interactions with cellular integrins, and inhibits cytolysis by the complement C5b-9 complex (2-4). Vitronectin also interacts with several critical proteins that regulate thrombosis and fibrinolysis. Vitronectin is phosphorylated by PKA and CKII (5).

Alternate Names

Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN

Gene Symbol

VTN

Additional Vitronectin Products

Product Documents for Vitronectin Antibody (1G11E8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Vitronectin Antibody (1G11E8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Customer Reviews for Vitronectin Antibody (1G11E8) - BSA Free

There are currently no reviews for this product. Be the first to review Vitronectin Antibody (1G11E8) - BSA Free and earn rewards!

Have you used Vitronectin Antibody (1G11E8) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies