VMAT2 Antibody (CL13157) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44388

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13157

Format

Azide and BSA Free
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Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q05940, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for VMAT2 Antibody (CL13157) - Azide and BSA Free

VMAT2 Antibody (CL13157) - Azide and BSA Free Immunohistochemistry-Paraffin: VMAT2 Antibody [NBP3-44388]

Immunohistochemistry-Paraffin: VMAT2 Antibody [NBP3-44388]

Staining of human dorsal raphe nucleus shows strong cytoplasmic positivity in neurons.
VMAT2 Antibody (CL13157) - Azide and BSA Free Immunohistochemistry-Paraffin: VMAT2 Antibody [NBP3-44388]

Immunohistochemistry-Paraffin: VMAT2 Antibody [NBP3-44388]

Staining of human cerebral cortex shows positivity in single neural fibers, and no staining in neurons as expected.
VMAT2 Antibody (CL13157) - Azide and BSA Free Immunohistochemistry-Paraffin: VMAT2 Antibody [NBP3-44388]

Immunohistochemistry-Paraffin: VMAT2 Antibody [NBP3-44388]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
VMAT2 Antibody (CL13157) - Azide and BSA Free Western Blot: VMAT2 Antibody [NBP3-44388]

Western Blot: VMAT2 Antibody [NBP3-44388]

Analysis in human cell line HEL.
VMAT2 Antibody (CL13157) - Azide and BSA Free Immunohistochemistry-Paraffin: VMAT2 Antibody [NBP3-44388]

Immunohistochemistry-Paraffin: VMAT2 Antibody [NBP3-44388]

Staining of human stomach shows strong cytoplasmic positivity in gastric endocrine cells.

Applications for VMAT2 Antibody (CL13157) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VMAT2

VMAT2 (Vesicular amine transporter 2) acts to accumulate cytosolic monoamines into synaptic vesicles. Its function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders such as dementia with Lewy bodies. The transporter is a site of action of important drugs, including reserpine and tetrabenazine.

Long Name

Vesicular Monoamine Transporter 2/Solute Carrier Family 18, Member 2

Alternate Names

MNAT, SLC18A2, SVAT, SVMT, VAT2

Gene Symbol

SLC18A2

Additional VMAT2 Products

Product Documents for VMAT2 Antibody (CL13157) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for VMAT2 Antibody (CL13157) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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FAQs

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