VPS24 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89391

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VPS24 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS24 Antibody - BSA Free

Immunohistochemistry-Paraffin: VPS24 Antibody [NBP1-89391]

Immunohistochemistry-Paraffin: VPS24 Antibody [NBP1-89391]

Immunohistochemistry-Paraffin: VPS24 Antibody [NBP1-89391] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
VPS24 Antibody - BSA Free Western Blot: VPS24 Antibody - BSA Free [NBP1-89391]

Western Blot: VPS24 Antibody - BSA Free [NBP1-89391]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for VPS24 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS24

Component of the escrt-iii complex, which is required for multivesicular bodies (mvbs) formation and sorting of endosomal cargo proteins into mvbs. the mvb pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. the escrt-iii complex is probably involved in the concentration of mvb cargo. required for sorting/trafficking of egf receptor. in case of infection, the hiv-1 virus takes advantage of the escrt-iii complex for budding and exocytic cargos of viral proteins.

Alternate Names

CGI-149, CHMP325.1 protein, Chromatin-modifying protein 3, hVps24, member 3,25.1, NEDFFLJ38114, vacuolar protein sorting 24 (yeast), vacuolar protein sorting 24 homolog (S. cerevisiae)

Gene Symbol

CHMP3

Additional VPS24 Products

Product Documents for VPS24 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for VPS24 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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