VRK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93500

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VRK1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1). GHLPWEDNLKDPKYVRDSKIRYRENIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSKDDGKLDLSVVENGGLKAKTITKKRKKEIEESKEPGVEDTEWSNTQTEEAIQTRSRTRKRVQK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VRK1 Antibody - BSA Free

Western Blot: VRK1 AntibodyBSA Free [NBP2-93500]

Western Blot: VRK1 AntibodyBSA Free [NBP2-93500]

Western Blot: VRK1 Antibody [NBP2-93500] - Analysis of extracts of various cell lines, using VRK1. Exposure time: 90s.
Immunohistochemistry-Paraffin: VRK1 Antibody - BSA Free [NBP2-93500]

Immunohistochemistry-Paraffin: VRK1 Antibody - BSA Free [NBP2-93500]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP2-93500] - Paraffin-embedded rat lung using VRK1.
Immunohistochemistry-Paraffin: VRK1 Antibody - BSA Free [NBP2-93500]

Immunohistochemistry-Paraffin: VRK1 Antibody - BSA Free [NBP2-93500]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP2-93500] - Paraffin-embedded mouse lung using VRK1.
VRK1 Antibody - BSA Free

Western Blot: VRK1 Antibody - BSA Free [NBP2-93500] -

Western Blot: VRK1 Antibody - BSA Free [NBP2-93500] - Western blot analysis of lysates from wild type(WT) and VRK1 knockout (KO) 293T cells, using [KO Validated] VRK1 Rabbit pAb at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Applications for VRK1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VRK1

VRK1 encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.

Long Name

Vaccinia-related Kinase 1

Alternate Names

EC 2.7.11.1, MGC117401, MGC138280, MGC142070, PCH1, serine/threonine-protein kinase VRK1, vaccinia related kinase 1, vaccinia virus B1R-related kinase 1, Vaccinia-related kinase 1

Gene Symbol

VRK1

Additional VRK1 Products

Product Documents for VRK1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for VRK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
p53 Pathway

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Protocols

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