VSIG1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81073

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KAKAKAKERNSKTIAELEPMTKINPRGESEAMPREDATQLEVTLPSSIHETGPDTIQEPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VSIG1 Antibody - BSA Free

Western Blot: VSIG1 Antibody [NBP1-81073]

Western Blot: VSIG1 Antibody [NBP1-81073]

Western Blot: VSIG1 Antibody [NBP1-81073] - Analysis in human cell line PC-3.
Immunocytochemistry/ Immunofluorescence: VSIG1 Antibody [NBP1-81073]

Immunocytochemistry/ Immunofluorescence: VSIG1 Antibody [NBP1-81073]

Immunocytochemistry/Immunofluorescence: VSIG1 Antibody [NBP1-81073] - Staining of human cell line PC-3 shows localization to cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073] - Staining in human stomach and liver tissues using anti-VSIG1 antibody. Corresponding VSIG1 RNA-seq data are presented for the same tissues.
Western Blot: VSIG1 Antibody [NBP1-81073]

Western Blot: VSIG1 Antibody [NBP1-81073]

Western Blot: VSIG1 Antibody [NBP1-81073] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073] - Staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81073] - Staining of human stomach shows high expression.

Applications for VSIG1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:10-1:20

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VSIG1

The VSIG1 gene encodes a member of the junctional adhesion molecule (JAM) family. The encoded protein contains multipleglycosylation sites at the N-terminal region, and multiple phosphorylation sites and glutamic acid/proline (EP)repeats at the C-terminal regi

Long Name

V-Set and Immunoglobulin Domain Containing 1

Alternate Names

GPA34

Entrez Gene IDs

340547 (Human)

Gene Symbol

VSIG1

UniProt

Additional VSIG1 Products

Product Documents for VSIG1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VSIG1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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FAQs

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