VSIG1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81074

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VSIG1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QAVAIGQFKDRITGSNDPGNASITISHMQPADSGIYICDVNNPPDFLGQNQGILNVSVLVKPSKPLCSVQGRPETGHTISLSCLSALGTPSPVYYWHKLEGRDIVPV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VSIG1 Antibody - BSA Free

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074] - Staining in human stomach and liver tissues using anti-VSIG1 antibody. Corresponding VSIG1 RNA-seq data are presented for the same tissues.
Western Blot: VSIG1 Antibody [NBP1-81074]

Western Blot: VSIG1 Antibody [NBP1-81074]

Western Blot: VSIG1 Antibody [NBP1-81074] - Analysis in control (vector only transfected HEK293T lysate) and vSIG1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074] - Staining of human liver shows low expression as expected.
Western Blot: VSIG1 Antibody [NBP1-81074]

Western Blot: VSIG1 Antibody [NBP1-81074]

Western Blot: VSIG1 Antibody [NBP1-81074] - Analysis in denatured VSIG1 overexpression lysate (Cat. # H00340547-T01, left), vector only transfected lysate (center) and native VSIG1 overexpression lysate (Cat. # NBL1-17761, right). Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074] - Staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.

Applications for VSIG1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-81074 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VSIG1

The VSIG1 gene encodes a member of the junctional adhesion molecule (JAM) family. The encoded protein contains multipleglycosylation sites at the N-terminal region, and multiple phosphorylation sites and glutamic acid/proline (EP)repeats at the C-terminal regi

Long Name

V-Set and Immunoglobulin Domain Containing 1

Alternate Names

GPA34

Entrez Gene IDs

340547 (Human)

Gene Symbol

VSIG1

UniProt

Q86XK7

Additional VSIG1 Products

Product Documents for VSIG1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VSIG1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Inflammatory Mediators

Citations for VSIG1 Antibody - BSA Free

Customer Reviews for VSIG1 Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • VSIG1 Antibody
    Name: Jennifer Cotton
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Paraffin sections of human and mouse intestine
    Species: Both human and mouse tissue
    Verified Customer | Posted 12/08/2016
    VSIG-1, IHC-Paraffin section of mouse tissue
    VSIG1 Antibody - BSA Free NBP1-81074
  • Name: Ming Lu
    Application: Western Blot
    Sample Tested: VSIG1 overepxression lysate
    Species: Human
    Verified Customer | Posted 09/26/2014
    VSIG1 NBP1-81074 blot
    VSIG1 Antibody - BSA Free NBP1-81074

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