VSV-G Epitope Tag Antibody - BSA Free

Novus Biologicals | Catalog # NB100-2485

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VSV-G Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide: YTDIEMNRLGK conjugated to KLH.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VSV-G Epitope Tag Antibody - BSA Free

Western Blot: VSV-G Epitope Tag AntibodyBSA Free [NB100-2485]

Western Blot: VSV-G Epitope Tag AntibodyBSA Free [NB100-2485]

Western Blot: VSV-G Epitope Tag Antibody [NB100-2485] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

Applications for VSV-G Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunoprecipitation

1 - 4 ug/mg lysate

Simple Western

1:30 - 1:270

Western Blot

1:1000-1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
See Simple Western Antibody Database for Simple Western validation: Tested in Multi-tag protein (ab36791), separated by Size, antibody dilution of 1:30, 1:90, 1:270, apparent MW was 54 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VSV-G Epitope Tag

The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized VSV-G epitope represents the amino acid sequence YTDIEMNRLGK derived from the Vesicular Stomatitis viral glycoprotein.

Alternate Names

Vesicular stomatitis virus glycoprotein tag, VSV epitope tag, VSV tag, VSVG, VSV-G epitope tag, vsvg tag

Additional VSV-G Epitope Tag Products

Product Documents for VSV-G Epitope Tag Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for VSV-G Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VSV-G Epitope Tag Antibody - BSA Free

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Protocols

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FAQs

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