Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Loading...
Product Specifications
Immunogen
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the central region of human WASF2. This sequence has high homology with similar regions in rat and mouse WASF2, and has low homology to similar regions in WAVE1 and WAVE3. [UniProt# Q9Y6W5]
Specificity
The antibody detects a 84 kDa protein corresponding to the molecular mass of WASF2 on SDS-PAGE immunoblots of human K562, mouse C2C12, and rat PC12 cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for WASF2 Antibody
Immunocytochemistry/Immunofluorescence: WASF2 Antibody [NBP3-23200] -
Immunocytochemistry/Immunofluorescence: WASF2 Antibody [NBP3-23200] - Immunocytochemical labeling of WASF2 in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal WASF2 (Central) antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.Western Blot: WASF2 Antibody [NBP3-23200] -
Western Blot: WASF2 Antibody [NBP3-23200] - Western blot of rat PC12 lysate. The blots were probed with anti-WASF2 (Central region) in the presence (lane 2) or absence (lane 1) of WASF2 (Central region) blocking peptide.Applications for WASF2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: WASF2
Alternate Names
IMD2, Protein WAVE-2, SCAR2suppressor of cyclic-AMP receptor (WASP-family), Verprolin homology domain-containing protein 2, WAS protein family, member 2, WASP family Verprolin-homologous protein 2, WAVE2dJ393P12.2, wiskWASP family protein member 2
Gene Symbol
WASF2
Additional WASF2 Products
Product Documents for WASF2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for WASF2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for WASF2 Antibody
There are currently no reviews for this product. Be the first to review WASF2 Antibody and earn rewards!
Have you used WASF2 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...