WASP Antibody (7B10E4) - BSA Free

Novus Biologicals | Catalog # NBP2-52587

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 7B10E4

Format

BSA Free
View all WASP Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human WASP (AA: 57-170) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WASP Antibody (7B10E4) - BSA Free

Western Blot: WASP Antibody (7B10E4)BSA Free [NBP2-52587]

Western Blot: WASP Antibody (7B10E4)BSA Free [NBP2-52587]

Western Blot: WASP Antibody (7B10E4) [NBP2-52587] - Analysis using WAS mAb against human WAS (AA: 57-170) recombinant protein. (Expected MW is 39 kDa)
Immunohistochemistry-Paraffin: WASP Antibody (7B10E4) - BSA Free [NBP2-52587]

Immunohistochemistry-Paraffin: WASP Antibody (7B10E4) - BSA Free [NBP2-52587]

Immunohistochemistry-Paraffin: WASP Antibody (7B10E4) [NBP2-52587] - Analysis of paraffin-embedded colon cancer tissues using WAS mouse mAb with DAB staining.
Flow Cytometry: WASP Antibody (7B10E4) - BSA Free [NBP2-52587]

Flow Cytometry: WASP Antibody (7B10E4) - BSA Free [NBP2-52587]

Flow Cytometry: WASP Antibody (7B10E4) [NBP2-52587] - Analysis of Hela cells using WAS mouse mAb (green) and negative control (red).
Western Blot: WASP Antibody (7B10E4)BSA Free [NBP2-52587]

Western Blot: WASP Antibody (7B10E4)BSA Free [NBP2-52587]

Western Blot: WASP Antibody (7B10E4) [NBP2-52587] - Analysis using WAS mAb against HEK293 (1) and WAS (AA: 57-170)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: WASP Antibody (7B10E4) - BSA Free [NBP2-52587]

Immunohistochemistry-Paraffin: WASP Antibody (7B10E4) - BSA Free [NBP2-52587]

Immunohistochemistry-Paraffin: WASP Antibody (7B10E4) [NBP2-52587] - Analysis of paraffin-embedded ovarian cancer tissues using WAS mouse mAb with DAB staining.
ELISA: WASP Antibody (7B10E4) - BSA Free [NBP2-52587]

ELISA: WASP Antibody (7B10E4) - BSA Free [NBP2-52587]

ELISA: WASP Antibody (7B10E4) [NBP2-52587] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for WASP Antibody (7B10E4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WASP

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.

Long Name

Wiskott-Aldrich Syndrome Protein

Alternate Names

Thrombocytopenia 1, WAS

Gene Symbol

WAS

Additional WASP Products

Product Documents for WASP Antibody (7B10E4) - BSA Free

Certificate of Analysis

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Product Specific Notices for WASP Antibody (7B10E4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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