WDR77 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-82778
![Western Blot: WDR77 Antibody [NBP1-82778]](https://resources.rndsystems.com/images/products/WDR77-Antibody-Western-Blot-NBP1-82778-img0006.jpg "Western Blot: WDR77 Antibody [NBP1-82778]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SEDCSIAVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for WDR77 Antibody - BSA Free
Western Blot: WDR77 Antibody [NBP1-82778]
Western Blot: WDR77 Antibody [NBP1-82778] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).![Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP1-82778]](https://resources.rndsystems.com/images/products/WDR77-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82778-img0008.jpg "Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP1-82778]")
Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP1-82778]
Immunocytochemistry/Immunofluorescence: WDR77 Antibody [NBP1-82778] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & the Golgi apparatus. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]](https://resources.rndsystems.com/images/products/WDR77-Antibody-Immunohistochemistry-Paraffin-NBP1-82778-img0013.jpg "Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]")
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778] - Staining of human rectum shows weak to moderate cytoplasmic and nuclear positivity in glandular cells.![Western Blot: WDR77 Antibody [NBP1-82778]](https://resources.rndsystems.com/images/products/WDR77-Antibody-Western-Blot-NBP1-82778-img0005.jpg "Western Blot: WDR77 Antibody [NBP1-82778]")
Western Blot: WDR77 Antibody [NBP1-82778]
Western Blot: WDR77 Antibody [NBP1-82778] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]](https://resources.rndsystems.com/images/products/WDR77-Antibody-Immunohistochemistry-Paraffin-NBP1-82778-img0009.jpg "Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]")
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778] - Staining of human fallopian tube shows cytoplasmic and nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]](https://resources.rndsystems.com/images/products/WDR77-Antibody-Immunohistochemistry-NBP1-82778-img0010.jpg "Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]")
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778] - Staining of human liver shows no positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]](https://resources.rndsystems.com/images/products/WDR77-Antibody-Immunohistochemistry-Paraffin-NBP1-82778-img0012.jpg "Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]")
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778]
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.Applications for WDR77 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: WDR77
Alternate Names
Androgen receptor cofactor p44, MEP-50, MEP50Nbla10071, methylosome protein 50, MGC2722, p44, p44/Mep50, RP11-552M11.3, WD repeat domain 77, WD repeat-containing protein 77
Gene Symbol
WDR77
Additional WDR77 Products
Product Documents for WDR77 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for WDR77 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for WDR77 Antibody - BSA Free
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Q: I have seen that the peptide from which was raised the antibody NBP1-82778 is 85% homologous in mice. My question is: is this antibody working for IHC also in mouse or not?
A:
NBP1-82778 has never been tested in mouse, however, since the immunogen shares 88% similarity with mouse, it is predicted to cross-react since it is a polyclonal antibody. You would be glad to learn that for untested species or applications, we offer our Innovator's Reward program. When you submit a review of your experiment, we will supply you with a credit for the purchase price of the antibody. Contact us for more information at innovators@novusbio.com.
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