WIPI1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88878

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WIPI1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HQDRAFATARLNFSGQRNICTLSTIQKLPRLLVASSSGHLYMYNLDPQDGGECVLIKTHSLLGSGTTEENKENDLRPSLPQSYAATVARPSASSASTVPGYSEDGGALRGEVIPEHEFATGPVCLDDENEFPPIILCRG

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26158518).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WIPI1 Antibody - BSA Free

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Analysis in human placenta and fallopian tube tissues using NBP1-88878 antibody. Corresponding WIPI1 RNA-seq data are presented for the same tissues.
Western Blot: WIPI1 Antibody [NBP1-88878]

Western Blot: WIPI1 Antibody [NBP1-88878]

Western Blot: WIPI1 Antibody [NBP1-88878] - Analysis in human cell lines SK-MEL-30 and HeLa using anti-WIPI1 antibody. Corresponding WIPI1 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.
Western Blot: WIPI1 Antibody [NBP1-88878]

Western Blot: WIPI1 Antibody [NBP1-88878]

Western Blot: WIPI1 Antibody [NBP1-88878] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular and enteroendocrine cells.
Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining of human fallopian tube shows very weak cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878]

Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic and decidual cells.

Applications for WIPI1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Simple Western

1:50-1:1000

Western Blot

1:100-1:500
Application Notes
WB reported in scientific literature (PMID: 26158518). For IHC-Paraffin, HIER pH 6 retrieval is recommended. WIPI1 antibody validated for Simple Western from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: Tested in mouse brain lysate, separated by Size, antibody dilution of 1:50 - 1:1000

Reviewed Applications

Read 1 review rated 4 using NBP1-88878 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WIPI1

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Alternate Names

Atg18, Atg18 protein homolog, ATG18A, FLJ10055, WD repeat domain phosphoinositide-interacting protein 1, WD repeat domain, phosphoinositide interacting 1, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WD40 repeat protein Interacting with phosphoInositides of 49kDa, WIPI 49 kDa, WIPI-1, WIPI-1 alpha, WIPI49ATG18

Gene Symbol

WIPI1

Additional WIPI1 Products

Product Documents for WIPI1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WIPI1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for WIPI1 Antibody - BSA Free

Customer Reviews for WIPI1 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Simple Western
    Sample Tested: mouse whole brain lysate
    Species: Mouse
    Verified Customer | Posted 03/25/2016
    WIPI1 expression in mouse brain lysate
    WIPI1 Antibody - BSA Free NBP1-88878

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Protocols

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