WIPI2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13110

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WIPI2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human WIPI2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for WIPI2 Antibody - BSA Free

Western Blot: WIPI2 Antibody [NBP3-13110]

Western Blot: WIPI2 Antibody [NBP3-13110]

Western Blot: WIPI2 Antibody [NBP3-13110] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WIPI2 antibody (NBP3-13110) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: WIPI2 Antibody [NBP3-13110]

Immunohistochemistry-Paraffin: WIPI2 Antibody [NBP3-13110]

Immunohistochemistry-Paraffin: WIPI2 Antibody [NBP3-13110] - WIPI2 antibody detects WIPI2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. WIPI2 antibody (NBP3-13110) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: WIPI2 Antibody [NBP3-13110]

Western Blot: WIPI2 Antibody [NBP3-13110]

Western Blot: WIPI2 Antibody [NBP3-13110] - Various whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with WIPI2 antibody (NBP3-13110) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
WIPI2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: WIPI2 Antibody [NBP3-13110] -

WIPI2 antibody detects WIPI2 protein at Preautophagosomal structure membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: WIPI2 protein stained by WIPI2 antibody (NBP3-13110) diluted at 1:2500.
Blue: Hoechst 33342 staining.

Applications for WIPI2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WIPI2

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Alternate Names

ATG18B, Atg21, CGI-50, DKFZP434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984, WD repeat domain phosphoinositide-interacting protein 2, WD repeat domain, phosphoinositide interacting 2, WD40 repeat protein interacting with phosphoinositides 2, WIPI-2, WIPI49-like protein 2

Gene Symbol

WIPI2

Additional WIPI2 Products

Product Documents for WIPI2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WIPI2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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