WWOX Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33456

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WWOX Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human WWOX. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus, Mitochondrion, Golgi apparatus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WWOX Antibody - BSA Free

Western Blot: WWOX Antibody [NBP1-33456]

Western Blot: WWOX Antibody [NBP1-33456]

Western Blot: WWOX Antibody [NBP1-33456] - Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with WWOX antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: WWOX Antibody [NBP1-33456]

Immunocytochemistry/ Immunofluorescence: WWOX Antibody [NBP1-33456]

Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP1-33456] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456] - WWOX antibody detects WWOX protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. WWOX antibody dilution: 1:500.
Western Blot: WWOX Antibody [NBP1-33456]

Western Blot: WWOX Antibody [NBP1-33456]

Western Blot: WWOX Antibody [NBP1-33456] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.
Western Blot: WWOX Antibody [NBP1-33456]

Western Blot: WWOX Antibody [NBP1-33456]

Western Blot: WWOX Antibody [NBP1-33456] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456] - WWOX antibody detects WWOX protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. WWOX antibody dilution: 1:100.
Immunoprecipitation: WWOX Antibody [NBP1-33456]

Immunoprecipitation: WWOX Antibody [NBP1-33456]

Immunoprecipitation: WWOX Antibody [NBP1-33456] - WWOX antibody immunoprecipitates WWOX protein in IP experiments. IP samples: 293T whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of WWOX protein by 4 µg WWOX antibody. 10 % SDS-PAGE. The immunoprecipitated WWOX protein was detected by WWOX antibody diluted at 1:500.

Applications for WWOX Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WWOX

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism. The encoded protein is more than 90% identical to the mouse protein, which is an essential mediator of tumor necrosis factor-alpha-induced apoptosis, suggesting a similar, important role in apoptosis for the human protein. In addition, there is evidence that this gene behaves as a suppressor of tumor growth. Alternative splicing of this gene generates transcript variants that encode different isoforms.

Long Name

WW Domain Containing Oxidoreductase

Alternate Names

FOR, FRA16D, SDR41C1, WOX1

Gene Symbol

WWOX

Additional WWOX Products

Product Documents for WWOX Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for WWOX Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

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Protocols

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