WWOX Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-47579

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence

Cited:

Immunoblotting

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WWOX Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VYYANHTEEKTQWEHPKTGKRKRVAGDLPYGWEQETDENGQVFFVDHINKRTTYLDPRLAFTVDDNPTKPTTRQRYDGS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WWOX Antibody - BSA Free

Western Blot: WWOX Antibody [NBP2-47579]

Western Blot: WWOX Antibody [NBP2-47579]

Western Blot: WWOX Antibody [NBP2-47579] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: WWOX Antibody [NBP2-47579]

Immunocytochemistry/ Immunofluorescence: WWOX Antibody [NBP2-47579]

Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP2-47579] - Staining of human cell line MCF7 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human thyroid gland shows strong cytoplasmic postivity in glandular cells.
Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human skeletal muscle shows no postivity in myoctyes as expected.
Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human kidney shows moderate cytoplasmic postivity in cells in tubules.
Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579]

Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in cells in molecular layer.
Immunohistochemistry: WWOX Antibody [NBP2-47579]

Immunohistochemistry: WWOX Antibody [NBP2-47579]

Immunohistochemistry: WWOX Antibody [NBP2-47579] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.

Applications for WWOX Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. iCC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in Immunoblotting reported in scientific literature (PMID: 28504702).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WWOX

WW domain-containing oxidoreductase (WWOX) is a proapoptotic protein and a tumor suppressor gene. Because mutation or deletion in the coding region of WWOX is rarely found, it is speculated that the appearance of aberrant transcripts affects progression of many cancers. In addition, protein levels of WWOX, its Tyr33-phosphorylated form, and WOX2 are significantly down-regulated in the neurons of Alzheimer's hippocampi.

Long Name

WW Domain Containing Oxidoreductase

Alternate Names

FOR, FRA16D, SDR41C1, WOX1

Gene Symbol

WWOX

Additional WWOX Products

Product Documents for WWOX Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for WWOX Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for WWOX Antibody - BSA Free

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Protocols

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FAQs

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