Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all XBP1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human XBP-1. The immunogen is located within the last 50 amino acids of XBP-1. Amino Acid Squence: LPAWRSSQRSTQKDPVPY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit XBP1 Antibody - BSA Free (NBP1-77252) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-XBP1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for XBP1 Antibody - BSA Free

Western Blot: XBP1 AntibodyBSA Free [NBP1-77252]

Western Blot: XBP1 AntibodyBSA Free [NBP1-77252]

Western Blot: XBP1 Antibody [NBP1-77252] - PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Western Blot: XBP1 AntibodyBSA Free [NBP1-77252]

Western Blot: XBP1 AntibodyBSA Free [NBP1-77252]

Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77252]

Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77252]

Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77252] - HepG2 cells with XBP-1 antibody at 2 ug/ml.

Applications for XBP1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Western Blot

0.5-2 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP1-77252 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: XBP1

X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

Long Name

X-box Binding Protein 1

Alternate Names

HTF, TREB5, XBP2

Gene Symbol

XBP1

UniProt

P17861 (Human)

Additional XBP1 Products

Product Documents for XBP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for XBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for XBP1 Antibody - BSA Free

Customer Reviews for XBP1 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used XBP1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • XBP1 Antibody
    Name: Wenliang Zhang
    Application: Western Blot
    Sample Tested: mouse liver
    Species: Mouse
    Verified Customer | Posted 02/09/2018
    Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation.
    XBP1 Antibody - BSA Free NBP1-77252

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies