XLF Antibody

Novus Biologicals | Catalog # NB100-53799

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all XLF Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QRPQLSKVKRKKPR corresponding to C-Terminus according to NP_079058.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for XLF Antibody

Western Blot: XLF Antibody [NB100-53799]

Western Blot: XLF Antibody [NB100-53799]

Western Blot: XLF Antibody [NB100-53799] - (0.1ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: XLF Antibody [NB100-53799]

Immunohistochemistry-Paraffin: XLF Antibody [NB100-53799]

Immunohistochemistry-Paraffin: XLF Antibody [NB100-53799] - 3.8ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for XLF Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in lysates of human Skin, testis and thyroid gland (calculated size of 33.3 kDa band according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human placenta shows nuclear staining in trophoblasts.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XLF

XLF (also known as Cernunnos) is a DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. It may serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini

Alternate Names

Cernunnos, non-homologous end-joining factor 1, nonhomologous end-joining factor 1, Protein cernunnos, XLFFLJ12610, XRCC4-like factor

Entrez Gene IDs

79840 (Human)

Gene Symbol

NHEJ1

UniProt

NP_079058.1 (Human)

Additional XLF Products

Product Documents for XLF Antibody

Certificate of Analysis

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Product Specific Notices for XLF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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