XPD Antibody (6T9K6)

Novus Biologicals | Catalog # NBP3-15704

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6T9K6 expressed in HEK293
View all XPD Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human XPD (P18074). ARGKVSEGIDFVHHYGRAVIMFGVPYVYTQSRILKARLEYLRDQFQIRENDFLTFDAMRHAAQCVGRAIRGKTDYGLMVFADKRFARGDKRGKLPRWIQEH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XPD Antibody (6T9K6)

Western Blot: XPD Antibody (6T9K6) [NBP3-15704]

Western Blot: XPD Antibody (6T9K6) [NBP3-15704]

Western Blot: XPD Antibody (6T9K6) [NBP3-15704] - Western blot analysis of extracts of various cell lines, using XPD antibody (NBP3-15704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
XPD Antibody (6T9K6)

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] -

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] - Immunohistochemistry analysis of XPD in paraffin-embedded mouse brain tissue using XPD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
XPD Antibody (6T9K6)

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] -

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] - Immunohistochemistry analysis of XPD in paraffin-embedded human colon carcinoma tissue using XPD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
XPD Antibody (6T9K6)

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] -

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] - Immunohistochemistry analysis of XPD in paraffin-embedded human colon tissue using XPD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
XPD Antibody (6T9K6)

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] -

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] - Immunohistochemistry analysis of XPD in paraffin-embedded human cervix cancer tissue using XPD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
XPD Antibody (6T9K6)

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] -

Immunohistochemistry: XPD Antibody (6T9K6) [NBP3-15704] - Immunohistochemistry analysis of XPD in paraffin-embedded rat kidney tissue using XPD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Applications for XPD Antibody (6T9K6)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XPD

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Xeroderma Pigmentosum D

Alternate Names

EM9, ERCC2

Gene Symbol

ERCC2

Additional XPD Products

Product Documents for XPD Antibody (6T9K6)

Certificate of Analysis

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Product Specific Notices for XPD Antibody (6T9K6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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