XPR1 Antibody (HL2631)
Novus Biologicals | Catalog # NBP3-48708
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2631
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human XPR1. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for XPR1 Antibody (HL2631)
Western Blot: XPR1 Antibody (HL2631) [NBP3-48708] -
Non-transfected (-) and transfected (+) unboiled HCT-116 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XPR1 antibody [HL2631] (NBP3-48708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: XPR1 Antibody (HL2631) [NBP3-48708] -
Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with XPR1 antibody [HL2631] (NBP3-48708) diluted at 1:1000 and competitor's antibody (Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with and does not endorse this product.
Western Blot: XPR1 Antibody (HL2631) [NBP3-48708] -
Boiled and unboiled mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XPR1 antibody [HL2631] (NBP3-48708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.Western Blot: XPR1 Antibody (HL2631) [NBP3-48708] -
Boiled and unboiled HCT116 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XPR1 antibody [HL2631] (NBP3-48708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: XPR1 Antibody (HL2631) [NBP3-48708] -
Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XPR1 antibody [HL2631] (NBP3-48708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: XPR1 Antibody (HL2631) [NBP3-48708] -
Boiled and unboiled mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with XPR1 antibody [HL2631] (NBP3-48708) diluted at 1:1000 and competitor's antibody (Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.
Immunocytochemistry/ Immunofluorescence: XPR1 Antibody (HL2631) [NBP3-48708] -
XPR1 antibody [HL2631] detects XPR1 protein at cell membrane by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPR1 stained by XPR1 antibody [HL2631] (NBP3-48708) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Applications for XPR1 Antibody (HL2631)
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: XPR1
Alternate Names
FLJ90308, Protein SYG1 homolog, SYG1xenotropic and polytropic retrovirus receptor, X3, Xenotropic and polytropic murine leukemia virus receptor X3, xenotropic and polytropic retrovirus receptor 1, XR, X-receptor
Gene Symbol
XPR1
Additional XPR1 Products
Product Documents for XPR1 Antibody (HL2631)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for XPR1 Antibody (HL2631)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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