XRCC4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89463

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all XRCC4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XRCC4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: XRCC4 Antibody [NBP1-89463]

Immunocytochemistry/ Immunofluorescence: XRCC4 Antibody [NBP1-89463]

Immunocytochemistry/Immunofluorescence: XRCC4 Antibody [NBP1-89463] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463]

Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463]

Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Analysis in human testis and liver tissues using NBP1-89463 antibody. Corresponding XRCC4 RNA-seq data are presented for the same tissues.
XRCC4 Antibody - BSA Free Immunohistochemistry-Paraffin: XRCC4 Antibody - BSA Free [NBP1-89463]

Immunohistochemistry-Paraffin: XRCC4 Antibody - BSA Free [NBP1-89463]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
XRCC4 Antibody - BSA Free Immunohistochemistry-Paraffin: XRCC4 Antibody - BSA Free [NBP1-89463]

Immunohistochemistry-Paraffin: XRCC4 Antibody - BSA Free [NBP1-89463]

Staining of human liver shows no positivity in hepatocytes as expected.
XRCC4 Antibody - BSA Free Immunohistochemistry-Paraffin: XRCC4 Antibody - BSA Free [NBP1-89463]

Immunohistochemistry-Paraffin: XRCC4 Antibody - BSA Free [NBP1-89463]

Staining of human kidney shows moderate nuclear positivity in cells in tubules.
XRCC4 Antibody - BSA Free Immunohistochemistry-Paraffin: XRCC4 Antibody - BSA Free [NBP1-89463]

Immunohistochemistry-Paraffin: XRCC4 Antibody - BSA Free [NBP1-89463]

Staining of human small intestine shows strong nuclear positivity in glandular cells.
XRCC4 Antibody - BSA Free Western Blot: XRCC4 Antibody - BSA Free [NBP1-89463]

Western Blot: XRCC4 Antibody - BSA Free [NBP1-89463]

Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for XRCC4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: XRCC4

XRCC4 is encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq]

Alternate Names

complementing defective, repair in Chinese hamster, X-ray repair complementing defective repair in Chinese hamster cells 4, X-ray repair cross-complementing protein 4

Gene Symbol

XRCC4

Additional XRCC4 Products

Product Documents for XRCC4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for XRCC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for XRCC4 Antibody - BSA Free

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Protocols

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