YAP1 Antibody

Novus Biologicals | Catalog # NBP1-46105

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Canine

Predicted:

Human (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all YAP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this YAP1 antibody maps to a synthectic peptide with the sequence of C-TYHSRDESTDS (NP_006097).

Epitope

internal region

Specificity

This antibody targets all isoforms of YAP1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for YAP1 Antibody

Western Blot: YAP1 Antibody [NBP1-46105]

Western Blot: YAP1 Antibody [NBP1-46105]

Western Blot: YAP1 Antibody [NBP1-46105] - MDCK cell lysate (35 ug protein in RIPA buffer). YAP1 antibody dilution at 0.5 ug/mL, 1 hr incubation. Detected by chemiluminescence. Observed molecular weight is 65 kDa.

Applications for YAP1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.5 - 2 ug/mL
Application Notes
WB: A customer has reported an approx. 70 kDa band observed in lysates of MDCK (calculated MW of 50.3 kDa band according to Dog XP_536601.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: YAP1

The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).

References

1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025

Long Name

Yes-associated Protein 1

Alternate Names

YAP2, YAP65, YKI, Yorkie Homolog

Entrez Gene IDs

10413 (Human)

Gene Symbol

YAP1

Additional YAP1 Products

Product Documents for YAP1 Antibody

Certificate of Analysis

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Product Specific Notices for YAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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