YARS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86891

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all YARS Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for YARS Antibody - BSA Free

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining in human cerebral cortex and skeletal muscle tissues using NBP1-86891 antibody. Corresponding YARS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human cerebral cortex, pancreas, skeletal muscle and testis using Anti-YARS antibody NBP1-86891 (A) shows similar protein distribution across tissues to independent antibody NBP1-86890 (B).
Western Blot: YARS Antibody [NBP1-86891]

Western Blot: YARS Antibody [NBP1-86891]

Western Blot: YARS Antibody [NBP1-86891] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891]

Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Applications for YARS Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: YARS

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.

Alternate Names

CMTDIC, EC 6.1.1, EC 6.1.1.1, Tyrosyl--tRNA ligase, tyrosyl-tRNA synthetase, tyrosyl-tRNA synthetase, cytoplasmic, TYRRS, YRStyrRS, YTStyrosine tRNA ligase 1, cytoplasmic

Gene Symbol

YARS1

Additional YARS Products

Product Documents for YARS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for YARS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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