YB1 Antibody (4F12) - Azide and BSA Free

Novus Biologicals | Catalog # H00004904-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F12

Format

Azide and BSA Free
View all YB1 Primary Antibodies »

Product Specifications

Immunogen

YBX1 (NP_004550, 51 a.a. ~ 139 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DKKVIATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPGGVPVQGSKYA

Specificity

YBX1 - nuclease sensitive element binding protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for YB1 Antibody (4F12) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01]

Immunocytochemistry/ Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01]

Immunocytochemistry/Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01] - Analysis of monoclonal antibody to YBX1 on HeLa cell. Antibody concentration 20 ug/ml.
ELISA: YB1 Antibody (4F12) [H00004904-M01]

ELISA: YB1 Antibody (4F12) [H00004904-M01]

ELISA: YB1 Antibody (4F12) [H00004904-M01] - Detection limit for recombinant GST tagged YBX1 is approximately 0.3ng/ml as a capture antibody.

Applications for YB1 Antibody (4F12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: YB1

The Y-box binding protein 1 (YB1) is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumor aggressiveness and belongs to the cold-shock domain family.

Alternate Names

BP-8, CBF-A, class II, Y box-binding protein I, CSDA2, CSDB, DBPB CCAAT-binding transcription factor I subunit A, EFI-A, Enhancer factor I subunit A, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP1 Y-box-binding protein 1, nuclease-sensitive element-binding protein 1, Y box binding protein 1, YB1 DNA-binding protein B, YB-1 Y-box transcription factor, YBX1

Entrez Gene IDs

4904 (Human)

Gene Symbol

YBX1

OMIM

154030 (Human)

UniProt

NP_004550 (Human)

Additional YB1 Products

Product Documents for YB1 Antibody (4F12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for YB1 Antibody (4F12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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