YY1 Antibody (6J6L1)

Novus Biologicals | Catalog # NBP3-15779

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6J6L1 expressed in HEK293
View all YY1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human YY1 (P25490). PGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for YY1 Antibody (6J6L1)

Western Blot: YY1 Antibody (6J6L1) [NBP3-15779]

Western Blot: YY1 Antibody (6J6L1) [NBP3-15779]

Western Blot: YY1 Antibody (6J6L1) [NBP3-15779] - Western blot analysis of extracts of various cell lines, using YY1 antibody (NBP3-15779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
YY1 Antibody (6J6L1)

Western Blot: YY1 Antibody (6J6L1) [YY1] -

Western Blot: YY1 Antibody (6J6L1) [YY1] - Western blot analysis of various lysates using YY1 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
YY1 Antibody (6J6L1)

Chromatin Immunoprecipitation: YY1 Antibody (6J6L1) [YY1] -

Chromatin Immunoprecipitation: YY1 Antibody (6J6L1) [YY1] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
YY1 Antibody (6J6L1)

Immunocytochemistry/ Immunofluorescence: YY1 Antibody (6J6L1) [YY1] -

Immunocytochemistry/ Immunofluorescence: YY1 Antibody (6J6L1) [YY1] - Confocal imaging of HeLa cells using YY1 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Applications for YY1 Antibody (6J6L1)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5μg antibody for 10μg-15μg of Chromatin.

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:400

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: YY1

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Long Name

Yin-Yang-1

Alternate Names

DELTA, NF-E1, UCRBP

Gene Symbol

YY1

Additional YY1 Products

Product Documents for YY1 Antibody (6J6L1)

Certificate of Analysis

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Product Specific Notices for YY1 Antibody (6J6L1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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