YY1 Antibody

Novus Biologicals | Catalog # NBP1-46218

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all YY1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 50 and 100 of human Yin and Yang 1 Protein using the numbering given in entry NP_003394.1 (GeneID 7528).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for YY1 Antibody

Western Blot: YY1 Antibody [NBP1-46218]

Western Blot: YY1 Antibody [NBP1-46218]

Western Blot: YY1 Antibody [NBP1-46218] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-YY1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. YY1 was also immunoprecipitated by rabbit anti-YY1 antibody NBP1-46217, which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: YY1 Antibody [NBP1-46218]

Immunohistochemistry-Paraffin: YY1 Antibody [NBP1-46218]

Immunohistochemistry-Paraffin: YY1 Antibody [NBP1-46218] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-YY1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for YY1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: YY1

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Long Name

Yin-Yang-1

Alternate Names

DELTA, NF-E1, UCRBP

Entrez Gene IDs

7528 (Human); 22632 (Mouse)

Gene Symbol

YY1

UniProt

P25490

Additional YY1 Products

Product Documents for YY1 Antibody

Certificate of Analysis

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Product Specific Notices for YY1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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