YY1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-20932
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human YY1. The exact sequence is proprietary.
Localization
Nucleus matrix
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for YY1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: YY1 Antibody [NBP2-20932]
Immunocytochemistry/Immunofluorescence: YY1 Antibody [NBP2-20932] - YY1 antibody detects YY1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: YY1 stained by YY1 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932]
Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932] - C2C12 xenograft, using YY1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932]
Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932] - 59T xenograft, using YY1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Western Blot: YY1 Antibody [NBP2-20932] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with YY1 antibody (NBP2-20932) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: YY1 Antibody [NBP2-20932] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with YY1 antibody (NBP2-20932) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: YY1 Antibody - BSA Free [NBP2-20932] -
Inhibition of PTEN with SF1670 promotes the expression of NF-kappa B, YY1, and Gp-170. (A) Western Blot representative of the amount of total protein in RS4;11 cells treated with SF1670; the results show differences in the expression of p-Akt and Gp-170 (** p < 0.01; *** p < 0.001), after treatment with the inhibitor of PTEN. (B) The expression of NF-kappa B and YY1 in the cell line used is modified after treatment with the inhibitor (* p < 0.05; ** p < 0.01); PTEN does not show changes in the level of protein expression when using the PTEN inhibitor, and HIF-1 alpha has no significant changes. (C) Expression of mRNA of YY1 is increased in RS4;11 cells after SF1670 treatment (*** p = 0.001 treatment vs. untreated). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39063014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: YY1 Antibody - BSA Free [NBP2-20932] -
High intake of omega -6 PUFA induces the expression of aggressiveness markers. (A) Representative photomicrographs and analysis of YY1 expression (* p < 0.05 and ** p < 0.01). (B) COX-2 immunostaining, * p < 0.05. (C) TGF-beta immunostaining (* p < 0.05) in the groups of mice fed with control diet, balanced diet, and diet rich in omega -6. Representative microphotographs of the expression of the different markers are shown on the left. On the right side, the quantification of the stains can be seen, the data represent the mean + SEM, n = 5. Scale bar: 20 um. (D) Analysis of YY1, (E) COX-2, and (F) VEGF-A mRNA in the groups of mice fed a balanced diet and a diet rich in omega -6. Data represent mean + SEM, n = 5, * p < 0.05, ** p < 0.001. Results of three independent experiments are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35682855), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for YY1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: YY1
Additional YY1 Products
Product Documents for YY1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for YY1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Related Research Areas
Citations for YY1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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