YY1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20932

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human YY1. The exact sequence is proprietary.

Localization

Nucleus matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for YY1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: YY1 Antibody [NBP2-20932]

Immunocytochemistry/ Immunofluorescence: YY1 Antibody [NBP2-20932]

Immunocytochemistry/Immunofluorescence: YY1 Antibody [NBP2-20932] - YY1 antibody detects YY1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: YY1 stained by YY1 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.
Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932]

Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932]

Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932] - C2C12 xenograft, using YY1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932]

Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932]

Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932] - 59T xenograft, using YY1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
YY1 Antibody - BSA Free

Western Blot: YY1 Antibody [NBP2-20932] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with YY1 antibody (NBP2-20932) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
YY1 Antibody - BSA Free

Western Blot: YY1 Antibody [NBP2-20932] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with YY1 antibody (NBP2-20932) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
YY1 Antibody - BSA Free

Western Blot: YY1 Antibody - BSA Free [NBP2-20932] -

Inhibition of PTEN with SF1670 promotes the expression of NF-kappa B, YY1, and Gp-170. (A) Western Blot representative of the amount of total protein in RS4;11 cells treated with SF1670; the results show differences in the expression of p-Akt and Gp-170 (** p < 0.01; *** p < 0.001), after treatment with the inhibitor of PTEN. (B) The expression of NF-kappa B and YY1 in the cell line used is modified after treatment with the inhibitor (* p < 0.05; ** p < 0.01); PTEN does not show changes in the level of protein expression when using the PTEN inhibitor, and HIF-1 alpha has no significant changes. (C) Expression of mRNA of YY1 is increased in RS4;11 cells after SF1670 treatment (*** p = 0.001 treatment vs. untreated). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39063014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
YY1 Antibody - BSA Free

Immunohistochemistry: YY1 Antibody - BSA Free [NBP2-20932] -

High intake of omega -6 PUFA induces the expression of aggressiveness markers. (A) Representative photomicrographs and analysis of YY1 expression (* p < 0.05 and ** p < 0.01). (B) COX-2 immunostaining, * p < 0.05. (C) TGF-beta immunostaining (* p < 0.05) in the groups of mice fed with control diet, balanced diet, and diet rich in omega -6. Representative microphotographs of the expression of the different markers are shown on the left. On the right side, the quantification of the stains can be seen, the data represent the mean + SEM, n = 5. Scale bar: 20 um. (D) Analysis of YY1, (E) COX-2, and (F) VEGF-A mRNA in the groups of mice fed a balanced diet and a diet rich in omega -6. Data represent mean + SEM, n = 5, * p < 0.05, ** p < 0.001. Results of three independent experiments are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35682855), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for YY1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: YY1

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq]

Long Name

Yin-Yang-1

Alternate Names

DELTA, NF-E1, UCRBP

Gene Symbol

YY1

UniProt

Additional YY1 Products

Product Documents for YY1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for YY1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for YY1 Antibody - BSA Free

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Protocols

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FAQs

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