ZAG Antibody (7I8N3)

Novus Biologicals | Catalog # NBP3-15426

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7I8N3 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ZAG (P25311). MVRMVPVLLSLLLLLGPAVPQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMET

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZAG Antibody (7I8N3)

Immunohistochemistry-Paraffin: ZAG Antibody (7I8N3) [NBP3-15426]

Immunohistochemistry-Paraffin: ZAG Antibody (7I8N3) [NBP3-15426]

Immunohistochemistry-Paraffin: ZAG Antibody (7I8N3) [NBP3-15426] - Immunohistochemistry of paraffin-embedded human colon carcinoma using ZAG Rabbit mAb (NBP3-15426) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ZAG Antibody (7I8N3)

ZAG Antibody (7I8N3) [NBP3-15426] -

Analysis of lysates from Hep G2 cells using Zinc-alpha 2-glycoprotein (ZAG/AZGP1) Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60 s.

Applications for ZAG Antibody (7I8N3)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZAG

Zinc Alpha 2 Glycoprotein is a versatile protein which has several important functions such as fertilization and lipid metabolism. It has also been associated with causing fat loss with some cancer types. This protein is known to have interaction with ITGAV, USP53, STK11, PRKAB2, and PRKAB1.

Long Name

Zinc alpha-2-Glycoprotein

Alternate Names

AZGP1, ZA2G, ZNGP1

Gene Symbol

AZGP1

Additional ZAG Products

Product Documents for ZAG Antibody (7I8N3)

Certificate of Analysis

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Product Specific Notices for ZAG Antibody (7I8N3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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