ZAP70 [p Tyr352] Antibody (GT1686) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13623

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1686

Format

Azide and BSA Free
View all ZAP70 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr319 of human ZAP70. The exact sequence is proprietary.

Modification

p Tyr 352

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ZAP70 [p Tyr352] Antibody (GT1686) - Azide and BSA Free

Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] detects ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) stained by ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Applications for ZAP70 [p Tyr352] Antibody (GT1686) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZAP70

Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Long Name

Zeta-chain [TCR] Associated Protein Kinase 70 kDa

Alternate Names

mrtle, mur, SRK, STD, TZK

Gene Symbol

ZAP70

Additional ZAP70 Products

Product Documents for ZAP70 [p Tyr352] Antibody (GT1686) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ZAP70 [p Tyr352] Antibody (GT1686) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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