ZC3H8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84661

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZC3H8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KSRSKDYDVYSDNDICSQESEDNFAKELQQYIQAREMANAAQPEESTKKEGVKDTPQAAKQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZC3H8 Antibody - BSA Free

Immunohistochemistry-Paraffin: ZC3H8 Antibody [NBP1-84661]

Immunohistochemistry-Paraffin: ZC3H8 Antibody [NBP1-84661]

Immunohistochemistry-Paraffin: ZC3H8 Antibody [NBP1-84661] - Staining of human rectum shows strong nuclear positivity in glandular cells.
ZC3H8 Antibody - BSA Free Western Blot: ZC3H8 Antibody - BSA Free [NBP1-84661]

Western Blot: ZC3H8 Antibody - BSA Free [NBP1-84661]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
ZC3H8 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ZC3H8 Antibody - BSA Free [NBP1-84661]

Chromatin Immunoprecipitation-exo-Seq: ZC3H8 Antibody - BSA Free [NBP1-84661]

ChIP-Exo-Seq composite graph for Anti-ZC3H8 (NBP1-84661) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for ZC3H8 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZC3H8

ZC3H8 acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds tothe 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Induces thymocyte apoptosis

Alternate Names

Fliz1, ZC3HDC8, zinc finger CCCH domain-containing protein 8, zinc finger CCCH type domain containing 8, zinc finger CCCH-type containing 8, zinc finger CCCH-type domain containing 8

Gene Symbol

ZC3H8

Additional ZC3H8 Products

Product Documents for ZC3H8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ZC3H8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ZC3H8 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review ZC3H8 Antibody - BSA Free and earn rewards!

Have you used ZC3H8 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies