ZEB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-68930

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZEB1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to ZEB1 (zinc finger E-box binding homeobox 1) The peptide sequence was selected from the N terminal of ZEB1. Peptide sequence NPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPT. The peptide sequence for this immunogen was taken from within the described region.

Marker

Mesenchymal Cells Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for ZEB1 Antibody - BSA Free

Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: ZEB1.
Immunohistochemistry: ZEB1 Antibody [NBP1-68930]

Immunohistochemistry: ZEB1 Antibody [NBP1-68930]

Immunohistochemistry: ZEB1 Antibody [NBP1-68930] - Human Capan1 cells (Pancreatic cancer cell line) Primary Antibody Dilution: 1 : 300 Secondary Antibody: Anti-rabbit-AlexaFluor-488 Secondary Antibody Dilution: 1 : 200Color/Signal.
Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930] - Lane 1: Control, Lane 2: ZEB1 morphants. Upper panel: ZEB1 blot; Bottom panel: tubulin blot
Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930]

Western Blot: ZEB1 Antibody [NBP1-68930] - 293T cells lysate, concentration 0.2-1 ug/ml.

Applications for ZEB1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Reviewed Applications

Read 1 review rated 3 using NBP1-68930 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Gene Symbol

ZEB1

UniProt

P37275

Additional ZEB1 Products

Product Documents for ZEB1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ZEB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ZEB1 Antibody - BSA Free (1)

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Customer Images

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  • Name: Samantha Garcia
    Application: Western Blot
    Sample Tested: zebrafish embryonic trunk at 3 days post fertilization
    Species: Other
    Verified Customer | Posted 11/05/2013
    lane 1: control, lane 2: zeb1 morphants. Upper panel: zeb1 blot; bottom panel: tubulin blot
    ZEB1 Antibody - BSA Free NBP1-68930

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Protocols

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FAQs for ZEB1 Antibody - BSA Free

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    • Q: I'm looking for an antibody that recognize zeb1 (zinc finger E-box binding homeobox 1) in zebrafish. In particular, I am interested in the detection by WB of zeb1a (in zebrafish there are two different isoforms). Do you have an antibody that is at least predicted to be reactive in zebrafish?
      A: Our ZEB1 antibody #NBP1-68930 would be the best choice for you as its immunogen sequence is 100% identical to Zebrafish Zeb1. Besides WB, our customers have reported the workability of this antibody in ICC-IF application also. If you would be interested in testing this novel species or this novel application, please take a look at our Innovators Reward Program.

    • Q: In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
      A: ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
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